Development and clinical evaluation of a microcentrifugal analyzer method for determining creatine kinase MB isoenzyme.

Direct reaction-rate determination of creatine kinase isoenzyme MB with a centrifugal analyzer, based on immunoinhibition.

New manual and automated method for determining activity of creatine kinase isoenzyme MB, by use of dithiothreitol: clinical applications.

The method is based on the selective activating capacity of dithiothreitol on creatine kinase isoenzyme MB, after isoenzyme MM is activated by glutathione. Isolated isoenzymes MM and MB of human and canine origin were assayed individually and in mixtures of known activities. When glutathione was present in the assay medium the activity of each isoenzyme could be measured individually, but gluta...

Use of creatine kinase MB isoenzyme for diagnosing myocardial infarction when total creatine kinase activity is high.

The usefulness of measuring creatine kinase MB isoenzyme for diagnosing myocardial infarction when activities of total creatine kinase are very high is unclear. We conducted a retrospective study in an urban hospital that serves a largely indigent population. We concentrated on 146 patients whose creatine kinase activity was greater than 1000 U/L (upper limit of normal: 165 U/L for women and 22...

Time changes of creatine kinase and creatine kinase-MB isoenzyme versus discrimination values in the diagnosis of acute myocardial infarction: what is the optimal method for displaying the data?

We wanted to determine the optimal method for displaying data for the clinical evaluation of patients with possible acute myocardial infarction. Our primary question was, are the time changes (slopes) of the enzyme tests better predictors of disease than the discrimination values, i.e., the cut-off points? We studied 152 patients with and 114 without a diagnosis of acute myocardial infarction i...

Diagnosis of myocardial infarction by serum isoenzyme analysis.

Serial monitoring of the serum isoenzyme patterns of creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) in patients suspected of acute myocardial infarction has become a highly sensitive and specific diagnostic method. The predictable evolution of isoenzyme patterns following infarction permits diagnosis and recognition of early stages, recovery stages and extension of infarction in t...